Shariff was born in Mumbai to Indian father and Persian-Bahraini mother and studied at St. Anne's High School, Bandra. There were rumours regarding her dating her Kahiin To Hoga co-star Rajeev Khandelwal. Neither of them has ever acknowledged being together. As Aamna stepped into movies, she was linked with her co-star from the film Aloo Chaat, Aftab Shivdasani.
When Shariff was in her second year of college, she began receiving modelling offers for brands such as Close-Up Toothpaste, Beetel Mobile phones, Emami Cosmetic Cream, Clearasil Skin Cream, Nescafe and many others. She also appeared in Kumar Sanu's "Dil Ka Alam Main Kya Bataun Tujhe" music video as well Falguni Pathak's "Yeh Kisne Jadoo Kiya," Abhijeet's "Chalney Lagin Hain Hawain," Daler Mehndi's "Eh Le 100 Rupiya" and Shael's "Nachana Kamal Tera."
Shariff made her acting debut in the daily "Kahiin To Hoga" where she played the protagonist. The show was a major success and is one of the most successful shows in Star Plus history, ranking #5 on Star Plus's top 10 shows of all time. Most of the credit for the show's success goes to the sizzling chemistry between Aamna Shariff and her co-star Rajeev Khandelwal. Currently she is playing the lead role in Set India's serial "Hongey Juda Na Hum" which is basically a very unique love story.
Shariff made her film debut in the 2009 Bollywood film Aloo Chaat opposite Aftab Shivdasani. She appeared in another film that same year with Shivdasani again, called Aao Wish Karein.Her latest film is Shakal Pe Mat Ja directed by Shubh Mukherjee.